Monoclonal Antibody ; lyophilized
WB, ICC (P)
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human NQO1.
Recombinant human NQO1
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 °C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
NQO1 (NADPH Qui acceptor Oxidoreductase 1; also named DTD and Menadione reductase) is a 31-33 kDa cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO1 serves as a cyto-protective molecule, reducing qui s and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteasome mediated protein turnover. Human NQO1 contains 274 amino acids. It contains an FMN reductase domain (aa 5-212) and two utilized Lys acetylation sites. NQO1 functions as a non-covalent homodimer. Full length human NQO1 shares 86% aa sequence identity with mouse NQO1
NCBI Gene ID: